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Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0846-x

Abstract: Nitrogen-cycling microbial communities in municipal WWTPs were characterized.Numbers of , and genes were quantified by MPN-PCR.CAS process retained diverse nitrogen cycling populations.This study characterized the quantity and diversity of nitrogen cycling genes in various processes ofwere more abundant than the and archaeal genes, respectively.

Keywords： DNA microarray analysis     Nitrogen cycling functional genes     Most probable number-polymerase chain reaction    

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Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 6,   Pages 797-805 doi: 10.1007/s11783-012-0405-z

Abstract: vegetated with alfalfa ( L.) to evaluate the rhizosphere effects on the soil microbial population and functionalIn total, 796, 731, and 379 functional genes were detected by microarray in the AR, FR, and NR, respectivelyHigher proportions of functional genes related to carbon degradation and organic remediation, were foundgenes.rhizosphere and that salinity was negatively correlated with the abundance of carbon and organic degradation genes

Keywords： crude oil-contaminated soil     phytoremediation     rhizosphere effects     rhizobox     functional genes    

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Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 41-45 doi: 10.1007/s11684-007-0008-4

Abstract: Functional cytokines can enhance dendritic cell anti-tumor immune responses.

Keywords： Functional     experiment     anti-tumor     dendritic     clinical    

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MANURE AND METROPOLES---A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 1,

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SOIL NITROGEN CYCLING AND ENVIRONMENTAL IMPACTS IN THE SUBTROPICAL HILLY REGION OF CHINA: EVIDENCE FROM

Frontiers of Agricultural Science and Engineering 2022, Volume 9, Issue 3,   Pages 407-424 doi: 10.15302/J-FASE-2022448

Abstract:

● Soil nitrogen fluxes and influencing factors were reviewed in the subtropical hilly regions.

Keywords： nitrogen cycling     soil nitrogen     nitrogen deposition     greenhouse gases emission     non-point source pollution    

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Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 1,   Pages 4-4 doi: 10.1007/s11783-021-1438-y

Abstract: Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs).However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system

Keywords： Antibiotic resistance genes     Metal resistance genes     Metagenomic analysis     Microplastics     Mariculture    

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Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes

Daniel GAONA,Alfredo VALAREZO

Frontiers of Mechanical Engineering 2015, Volume 10, Issue 3,   Pages 294-300 doi: 10.1007/s11465-015-0351-0

Abstract:

The elastic modulus of a deposit (Ed) can be obtained by monitoring the temperature (?T) and curvature (?k) of a one-side coated long plate, namely, a one-dimensional (1D) deformation model. The aim of this research is to design an experimental setup that proves whether a 1D deformation model can be scaled for complex geometries. The setup includes a laser displacement sensor mounted on a robotic arm capable of scanning a specimen surface and measuring its deformation. The reproducibility of the results is verified by comparing the present results with Stony Brook University Laboratory’s results. The ?k-?T slope error is less than 8%, and the Ed estimation error is close to 2%. These values reveal the repeatability of the experiments. Several samples fabricated with aluminum as the substrate and 100MXC nanowire (Fe and Cr alloy) as the deposit are analyzed and compared with those in finite element (FE) simulations. The linear elastic behavior of 1D (flat long plate) and 2D (squared plate) specimens during heating/cooling cycles is demonstrated by the high linearity of all ?k-?T curves (over 97%). The Ed values are approximately equal for 1D and 2D analyses, with a median of 96 GPa and standard deviation of 2 GPa. The correspondence between the experimental and simulated results for the 1D and 2D specimens reveals that deformation and thermal stress in coated specimens can be predicted regardless of specimen geometry through FE modeling and by using the experimental value of Ed. An example of a turbine-blade-shaped substrate is presented to validate the approach.

Keywords： in-plane     Young’s modulus     curvature temperature     thermal stress     coating    

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MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 1,   Pages 182-187 doi: 10.15302/J-FASE-2020364

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Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1127-2

Abstract:

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

Keywords： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic    

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Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 6, doi: 10.1007/s11783-021-1415-5

Abstract:

• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.

Keywords： Antibiotic resistance genes     Organic solid waste     Aerobic composting     Livestock manure     Sludge    

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ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1521-z

Abstract:

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

Keywords： UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

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biomass-derived carbon loaded with MnO as lithium-ion battery anode for improving its reversible capacity and cycling

Frontiers of Chemical Science and Engineering 2023, Volume 18, Issue 1, doi: 10.1007/s11705-023-2376-y

Abstract: However, improving the reversible capacity and cycling performance remains a long-standing challenge.high current density of 1.0 A·g–1, KAC@MnO2 still exhibits excellent long-term cyclinganode materials, the KAC@MnO2 prepared in this work shows superior reversible capacity and cycling

Keywords： biomass-derived carbon     MnO2     lithium-ion batteries     anode material     high reversible capacity    

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Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 3, doi: 10.1007/s11783-021-1469-4

Abstract:

• Distribution of ARGs in decentralized sewage facilities were investigated.

Keywords： Decentralized sewage treatment facilities     Antibiotic resistance genes     Virus     Metagenomics     Network    

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Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 6, doi: 10.1007/s11783-021-1426-2

Abstract:

• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.

Keywords： Ammonia oxidizing archaea (AOA)     Complete ammonia oxidizing (comammox) bacteria     Dissimilatory nitrate reduction to ammonium (DNRA) bacteria     Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms     Engineering application    

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Development and applications of functional gene microarrays in the analysis of the functional diversity

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 1-20 doi: 10.1007/s11783-011-0301-y

Abstract: Functional gene arrays (FGAs) are a special type of microarrays containing probes for key genes involvedin microbial functional processes, such as biogeochemical cycling of carbon, nitrogen, sulfur, phosphorusSeveral generations of GeoChip have been developed and applied to investigate the functional diversity

Keywords： functional gene arrays (FGAs)     GeoChip     microbial communities     functional diversity/composition/structure    

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Title Author Date Type Operation

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

Journal Article

Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

Journal Article

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

Journal Article

MANURE AND METROPOLES---A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Journal Article

SOIL NITROGEN CYCLING AND ENVIRONMENTAL IMPACTS IN THE SUBTROPICAL HILLY REGION OF CHINA: EVIDENCE FROM

Journal Article

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

Journal Article

Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes

Daniel GAONA,Alfredo VALAREZO

Journal Article

MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF

Journal Article

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

Journal Article

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

Journal Article

ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

Journal Article

biomass-derived carbon loaded with MnO as lithium-ion battery anode for improving its reversible capacity and cycling

Journal Article

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized

Journal Article

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

Journal Article

Development and applications of functional gene microarrays in the analysis of the functional diversity

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

Journal Article